Plaque Formation by and Plaque Cloning of Chlamydia trachomatis Biovar Trachoma

Plaque formation by and plaque cloning of Chlamydia trachomatis biovar trachoma.

A new technique for the induction of plaque formation by Chlamydia trachomatis biovar trachoma applicable to the titration of infectivity and cloning of biovar trachoma was established. Three novel strains were cloned and confirmed to be free of glycogen inclusions. The lack of glycogen accumulation correlated with the absence of a 7.5-kb plasmid, which is highly conserved in other strains of C...

Plaque formation by Chlamydia in L cells.

Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg ld(50) determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-...

Chlamydia pneumoniae and atherosclerotic plaque.

To the Editor-According to Campbell et al. [1] in their recent study of Chlamydia pneumoniae in coronary tissue, staining with a Chlamydia-specific monoclonal antibody detected chlamydiae in 45% of the atherectomy specimens from patients with atherosclerotic plaque. However, staining appeared only in macrophages; smooth muscle cells did not stain, a result that appears to contradict previous fi...

Association of Chlamydia pneumoniae Infection With Atherosclerotic Plaque Formation.

Atherosclerosis is a complex multifactorial disorder. Studies show that infectious microbial agents may play an important role in the development of atherosclerosis; however, these findings are conflicting. This study investigated the presence of Chlamydia pneumoniae DNA in atherosclerotic plaques of patients suffering from coronary artery disease. In a cross-sectional study, 85 patients (43 fe...

The Formation of Plaques by African Horse Sickness Viruses and Factors Affecting Plaque Size